Journal: Histochemistry and Cell Biology

Article Title: EBF1 is expressed in pericytes and contributes to pericyte cell commitment

doi: 10.1007/s00418-021-02015-7

Figure Lengend Snippet: Primary antibodies used in the study

Article Snippet: Mouse monoclonal anti-SMA , Biocare Medical , CM 001 A.

Techniques:

Journal: Cells

Article Title: Depletion of Bone Marrow-Derived Fibrocytes Attenuates TAA-Induced Liver Fibrosis in Mice

doi: 10.3390/cells8101210

Figure Lengend Snippet: The antifibrotic effect was not accompanied by a reduction of myofibroblasts. ( a ) Western blot analysis and optical densitometry thereof revealed that the hepatic α-SMA levels were increased following TAA-treatment but unchanged by fibrocyte ablation. Two individual western blots were included in the analysis, a representative blot is shown. Arbitrary unit. SC n = 2; Ctrl, FC-Abl. n = 6. Mean + SEM is depicted. ( b ) Immunohistochemical staining of α-SMA (brown) demonstrated the periportal accumulation of myofibroblasts in TAA-treated animals and an unchanged expression pattern in result of the fibrocyte ablation. Representative stainings are shown. Magnification 40× and 200×, bars 400 and 50 µm. ( c ) Hepatic gene expression levels of Acta2 , Tgfb, and Pdgfb were comparable at the end of the experiment (full data in ).

Article Snippet: Western blot experiments were performed as described previously [ ] using 1:1.000 diluted antibodies against α-SMA (Mouse anti α-SMA monoclonal antibody, 61001, Progen, Heidelberg, Germany), Bax (Rabbit anti Bax polyclonal antibody, #2772), and Bcl-2 (Rabbit anti Bcl-2 polyclonal antibody, #2876).

Techniques: Western Blot, Immunohistochemical staining, Staining, Expressing, Gene Expression